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Reference impedance to be used while extracting scattering parameters of differential trace

Question asked by 9dc1212f-cb89-8a24-0100-014e727abccf on Jun 15, 2016

While extracting s parameters of differential line it asks for reference impedance, i provided 50 ohms for 100 ohm differential trace

If it is a 90 ohm differential trace should i provide 45 ohms or 50 ohms for reference impedance while extracting s parameters.

Will it cause any difference?